RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
5 to 10X enhancement of CRISPR-Cas9 editing efficiency with partially single-stranded DNA donors @craigedit https://t.co/akxBlXWPtF
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @ScientifixAU: NEW CRISPR protocol: https://t.co/vBysPo3SoH Please share with your friends and let me know how the hybrid donors work fo…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
Very excited to try this protocol. We've never had much luck with GFP-sized edits using linear repair templates. https://t.co/iSL5YCWEVA
RT @ScientifixAU: NEW CRISPR protocol: https://t.co/vBysPo3SoH Please share with your friends and let me know how the hybrid donors work fo…
NEW CRISPR protocol: https://t.co/vBysPo3SoH Please share with your friends and let me know how the hybrid donors work for you! The idea is two PCR products one with homology arms, one without, mix melt and anneal. The hybrids improve efficiency of GFP-s
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
@biorxivpreprint has Craig Mello @craigedit listed as first author. #uploadoops https://t.co/4aCtwKQ4cD
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @kavehdaneshvar: 1) Cas9 RNPs are toxic at high concentrations, 2) partially single-stranded dsDNA donors increase homology-directed rec…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @mjuette: Intriguing: recombination efficiencies as good as ssODNs, but for long (~kbp) inserts by using hybrid, asymmetric PCR donors.…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @kavehdaneshvar: 1) Cas9 RNPs are toxic at high concentrations, 2) partially single-stranded dsDNA donors increase homology-directed rec…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @kavehdaneshvar: 1) Cas9 RNPs are toxic at high concentrations, 2) partially single-stranded dsDNA donors increase homology-directed rec…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @kavehdaneshvar: 1) Cas9 RNPs are toxic at high concentrations, 2) partially single-stranded dsDNA donors increase homology-directed rec…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
1) Cas9 RNPs are toxic at high concentrations, 2) partially single-stranded dsDNA donors increase homology-directed recombination, and 3) Craig Mello posts pre-prints on BioRxiv. All very important information to me. https://t.co/yKCdFaUfrg
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
Top #tweeted #RNA list story: Robust genome editing with short single-stranded … https://t.co/d3mob74Q0R, see more https://t.co/QRe3vIMZc3
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @OdedRechavi: A new crispr protocol from Craig Mello’s lab - we must try this! https://t.co/KU3CYDytQs
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @jyouhou_syusyu: RT: Robust genome editing with short single-stranded and long, partially single-stranded DNA donors in C. elegans https…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @mjuette: Intriguing: recombination efficiencies as good as ssODNs, but for long (~kbp) inserts by using hybrid, asymmetric PCR donors.…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @millerlab: This sounds cool! https://t.co/Ffj7Y3iTsq
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @OdedRechavi: A new crispr protocol from Craig Mello’s lab - we must try this! https://t.co/KU3CYDytQs
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…
RT @craigedit: NEW CRISPR protocol: https://t.co/xCXKKYmtbr Please share with your friends and let me know how the hybrid donors work for y…