RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
RT @DNA_RNA_Uni: Hot #CRISPR preprint paper 🆕 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPRCas9-Mediate…
RT @DNA_RNA_Uni: Hot #CRISPR preprint paper 🆕 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPRCas9-Mediate…
RT @DNA_RNA_Uni: Hot #CRISPR preprint paper 🆕 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPRCas9-Mediate…
RT @DNA_RNA_Uni: Hot #CRISPR preprint paper 🆕 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPRCas9-Mediate…
Hot #CRISPR preprint paper 🆕 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPRCas9-Mediated Genome Editing Technology (2019) https://t.co/WV7UM6RSk7 @biorxivpreprint @CRISPRGuru #preprint #openaccess #retweet #DNARNA @gra
Re-Evaluating One-step Generation of #Mice Carrying Conditional Alleles by #CRISPR -Cas9-Mediated #Genome Editing Technology | bioRxiv @guruccb @rush6782 @JunieWarrington @apstrainees https://t.co/O7X8JMCpLh
@gwern @salonium or things like https://t.co/YAzMLBoW6E giving <1% Efficiency can get higher than 66% or close to 100% or have this very low results, some of the is Cas9 vs. other proteins, sometimes it's sequence to edit, sometimes protein-binding to
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
"Our analysis of a large data set indicates that the 2sgRNA–2ssODN method generates a large number of undesired alleles (>99%), and a very small number of desired alleles (<1%) requiring, on average 1,192 zygotes." #bioethics #CRISPR-Cas9 https://t.
RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
So true!
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
"You get what you look for - Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/BaDbtzS2hM
RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
😱 "The dataset constituted 17,887 microinjected or electroporated zygotes and 1,718 live born mice, of which only 15 (0.87%) mice harbored 2 correct LoxP insertions in cis configuration indicating a very low efficiency of the method."
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/H5yf6Y9e03
RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
@AnthonyAngueira
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @DesktopGenetics: A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mi…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @ivanoamelio: Feeling and mood regarding CRISPR/Cas9 is slowly slowly changing... I am not surprised! https://t.co/iBVImG4FG3
RT @ivanoamelio: Feeling and mood regarding CRISPR/Cas9 is slowly slowly changing... I am not surprised! https://t.co/iBVImG4FG3
Feeling and mood regarding CRISPR/Cas9 is slowly slowly changing... I am not surprised!
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
Interesting ...
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
A massive collaboration lead by @unmc analysed 17,887 microinjected or electroporated #zygotes and 1,718 live born #mice, discovering that the 2sgRNA–2ssODN method in #CRISPR experiments generates a large number of undesired alleles: https://t.co/ZCPydHrea
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
Who said CRISPR is easy?
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
Pretty sure that people saying CrispR is easy aren’t actually doing mouse generation...
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
It seems like there is room to improve from a cell line to a embryo.
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
RT @StearnsLab: As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as i…
As everyone trying to use CRISPR in mammalian cells other than HEK293 cells has realized, it is not as straightforward as is often advertised. https://t.co/7bQzSgtRxr
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @PaulHumpa: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology htt…
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/dJPEiVPFSt
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/BCSdtVzI0r
Researchers from 17 institutions around the world pooled their results regarding the use of #CRISPR to make conditional (loxP) knockout #mousemodels. They found that 0.87% of mice were correctly targeted. https://t.co/saQ3bm1Sh1
RT @smoltblue: ここまで否定された方法を今更試す気にはならないけれども畑田・堀居法の応用で2-step iGONADは試してみたい気もする。 Re-Evaluating One-step Generation of Mice Carrying Conditiona…
RT @smoltblue: ここまで否定された方法を今更試す気にはならないけれども畑田・堀居法の応用で2-step iGONADは試してみたい気もする。 Re-Evaluating One-step Generation of Mice Carrying Conditiona…
RT @smoltblue: ここまで否定された方法を今更試す気にはならないけれども畑田・堀居法の応用で2-step iGONADは試してみたい気もする。 Re-Evaluating One-step Generation of Mice Carrying Conditiona…
ここまで否定された方法を今更試す気にはならないけれども畑田・堀居法の応用で2-step iGONADは試してみたい気もする。 Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/NigfKFwcxD
RT @GLim2016: Interesting and helpful for all https://t.co/ocaV8dLXwr
Great community effort to show that 2sgRNA+2ssODN approach in one-step generation of conditional alleles in mouse is not so trivial!
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
RT @KeavneyLab: We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less ef…
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
RT @KeavneyLab: We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less ef…
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @KeavneyLab: We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less ef…
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Generation of conditional knock-out mouse with #CRISPR is less effective than previously thought.. less than 1%! 😬 https://t.co/xGFgWoP7Jy
👍🏻
RT @KeavneyLab: We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less ef…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @scekker: This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to mak…