This is such an important paper and a terrific example of #openscience. I cited it yesterday when I was told ‘it’s easy to make mouse cre/lox alleles using CRISPR, what’s wrong with #zebrafish?’
RT @KeavneyLab: We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less ef…
We're proud to have contributed to this work, showing that generation of conditional knock-out mouse with CRISPR is less effective than previously thought.
RT @YosukeKumamoto: Hmm... <1% of mice have correct loxP sites when targeted with 1step CRISPR. |Re-Evaluating One-step Generation of Mice…
RT @YosukeKumamoto: Hmm... <1% of mice have correct loxP sites when targeted with 1step CRISPR. |Re-Evaluating One-step Generation of Mice…
Hmm... <1% of mice have correct loxP sites when targeted with 1step CRISPR. |Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/pQhefqSCBh
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @druoh: 2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・ https://t.co/L5qBA01Ewp
Ouch for the CRISPR-Cas9 hype: "...2sgRNA–2ssODN method generates a large number of undesired alleles (>99%), and a very small number of desired alleles (<1%) requiring, on average 1,192 zygotes" https://t.co/l7XzTCtZ8G
RT @yamagatm3: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology htt…
RT @L_I_B: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology | bioRx…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @L_I_B: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology | bioRx…
RT @druoh: 2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・ https://t.co/L5qBA01Ewp
笑えね Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/Aic4Ic5v25
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @druoh: 2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・ https://t.co/L5qBA01Ewp
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/glfIiWT3nI
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @druoh: 2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・ https://t.co/L5qBA01Ewp
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @druoh: 2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・ https://t.co/L5qBA01Ewp
RT @CathyAbbottLab: Wow, fantastic community effort that will save a lot of wasted effort and animals. Pretty stunning discrepancy with ini…
RT @CathyAbbottLab: Not only a tour de force, but this thread is also a masterclass is using Twitter to communicate a complex set of result…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
2sgRNA–2ssODNでloxp挿入を行った17,887回のinjection/electroporationで1,718マウスが生まれ、2 correct loxpを持っているのは15匹・・・
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology | bioRxiv https://t.co/a6X93kv0kH
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @CRISPR_papers: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @jcornlab: Ouch! Not good news for the fizziness and hype problem of some early #crispr papers. https://t.co/mG49EN2QRi
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Ouch! Not good news for the fizziness and hype problem of some early #crispr papers.
Interesting and helpful for all
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @wthomp99: Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 lab…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Amazing work by @GaetanBurgio & @guruccb showing utility of 2sgRNA, 2ssODN CRISPR to produce conditional KO. Data from 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born...only 0.86% w/ 2 correct LoxP in Cis. Grateful to be able to contribute
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
"...the 2sgRNA–2ssODN method generates a large number of undesired alleles (>99%), and a very small number of desired alleles (<1%)..." Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Tech
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @usymmons: This is a fascinating thread abt trying to reproduce making conditional KOs in mice using CRISPR - bottom line: it's not triv…
RT @minu0812: CRISPR/Cas9を使っても一度の遺伝子導入でfloxアレルが作れる率は0.86%。。ここを解決できるとさらに一段便利になるはず。 https://t.co/OcSx6lJOyD
CRISPR/Cas9を使っても一度の遺伝子導入でfloxアレルが作れる率は0.86%。。ここを解決できるとさらに一段便利になるはず。
RT @NicolasDenans: We need more of this kind of studies. We always get single papers where they show how incredibly efficient their methods…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Thanks for putting this together and sharing.
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @evolvwing: Amazing... read this thread! #CRISPR #DNAcrobatics https://t.co/yGIZ0uuIm9
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @da40cambs: Woah. See preprint and thread https://t.co/uyv1yxEu8G
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @martpal: Thread - incredible body of work showing #CRISPR ing is not easy as some might think 🙏 https://t.co/VApp7AA4JT
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
Thread - incredible body of work showing #CRISPR ing is not easy as some might think 🙏
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @JayLVivian: Our experience has been very similar. The field needs to share, if nothing else, misery loves company. The initial anecd…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @JayLVivian: Our experience has been very similar. The field needs to share, if nothing else, misery loves company. The initial anecd…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @AMartinezArias: What a thread!....I can think of one or two experiments/statements where similar studies would reveal that things are n…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/7GjZAtitW0
RT @AMartinezArias: 17 labs, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 0.86% harbored correct LoxP https://t.co/OGnoHmBYH…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…