RT @JayLVivian: Our experience has been very similar. The field needs to share, if nothing else, misery loves company. The initial anecd…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Overestimation of effect size/method efficiency in initial papers is a classic #science outcome due to pressures to publish 'amazing stories' and no published negative data from others in the community. Thank you, @GaetanBurgio, for getting this out - so m
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
CRISPR is dead, it doesn't work? Totally useless?
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @LluisMontoliu: This collaborative effort led by @guruccb & @GaetanBurgio illustrates the power of sharing data to document difficulties…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @LluisMontoliu: This collaborative effort led by @guruccb & @GaetanBurgio illustrates the power of sharing data to document difficulties…
RT @LluisMontoliu: This collaborative effort led by @guruccb & @GaetanBurgio illustrates the power of sharing data to document difficulties…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @LluisMontoliu: This collaborative effort led by @guruccb & @GaetanBurgio illustrates the power of sharing data to document difficulties…
This collaborative effort led by @guruccb & @GaetanBurgio illustrates the power of sharing data to document difficulties in methods. A must read paper. Creating LoxP conditional mutant alleles with #CRISPR tools is less efficient than previously report
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @CathyAbbottLab: Not only a tour de force, but this thread is also a masterclass is using Twitter to communicate a complex set of result…
The initial anecdotal reports placed ultimately unrealistic expectations on efficiencies of making certain mutations, especially floxed alleles. Cores need to communicate this reality to their users. Plenty of room for improvement!
RT @CathyAbbottLab: Wow, fantastic community effort that will save a lot of wasted effort and animals. Pretty stunning discrepancy with ini…
Our experience has been very similar. The field needs to share, if nothing else, misery loves company. The initial anecdotal reports placed very high and ultimately unrealistic expectations on efficiencies of making flexed alleles
RT @CathyAbbottLab: Not only a tour de force, but this thread is also a masterclass is using Twitter to communicate a complex set of result…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @CathyAbbottLab: Not only a tour de force, but this thread is also a masterclass is using Twitter to communicate a complex set of result…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Not only a tour de force, but this thread is also a masterclass is using Twitter to communicate a complex set of results with great clarity.
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Wow, fantastic community effort that will save a lot of wasted effort and animals. Pretty stunning discrepancy with initial report...
RT @GaetanBurgio: Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 1…
Proud to share our community effort on the generation of conditional knockout mice using #CRISPR with 2sgRNA- 2ssODN -> 17 labs across US, Europe, Japan, Australia, 56 genes, 17,887 injected zygotes, 1,718 mice born => only 15 (0.86%) harbored correc
Top #tweeted #RNA list story: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology | bioRxiv https://t.co/TXlzzx8dPf, see more https://t.co/QRe3vIMZc3
RT @CRISPR_papers: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology…
RT @CRISPR_papers: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology…
RT @biorxivpreprint: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technolo…
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/NNqs6jyVjQ
RT @biorxivpreprint: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technolo…
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/rrQ9RNFPTG
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/HytDEFia3O
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/8aO7sQYGGy #biorxiv_genetic
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology https://t.co/L7zRUNCdDO #bioRxiv